We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Experimental Study of Cerebral Vasospasm Induced by Serotonin and Oxyhemoglobin in Subarachnoid Hemorrhage.
- Authors
Jiang Kun; Li Shao Hua; Bao Yao Dong
- Abstract
To clarify the mechanism of cerebral vasospasm induced by serotonin (5-HT) and oxyhemoglobin (OxyHb), the authors studied the roles of vasoactive prostaglandins (PGs) and free radicals in the cerebral vascular wall of rabbits. At first, they measured the 5-HT level in whole blood plus cerebrospinal fluid (CSF) and in platelet-rich plasma (PRP) plus CSF by fluorescence following the incubation at 37°C for zero to six days. The 5-HT in whole blood plus CSF did not significantly diminish for up to five days (p < 0.05), but in PRP plus CSF it markedly decreased within twenty-four hours (p < 0.01). The authors prepared 49 rabbits, divided into four groups: a nontreated (control) group and three groups treated with 5-HT, with OxyHb, and with free radical scavengers plus OxyHb, respectively. After treating the vessels with 5-HT or OxyHb, they measured levels of 6-keto-PGF1alpha; and thromboxane B2 (TXB2), the stable metabolites of prostacydin (PGI2) and TXA2, respectively, with PGE2 in vessels by radioimmunoassay. Vascular lipid peroxidation (LPO) was also measured by fiuorescence. In the nontreated group, the level of 6-keto-PGF1alpha; was significantly higher in the basilar vessels than that of any peripheral vessels (p < 0.01). In the group treated with 5-HT, 5-HT contracted both basilar and femoral arteries, but there was no significant change in levels of 6-keto-PGF1alpha;, TXB2, PGE2, and LPO. Vasospasm induced by 5-HT is not specific to the cerebral artery and does not depend on PGs and LPO. In the group treated with OxyHb, however, OxyHb induced severe vasospasm in the basilar vessel alone, where LOP was highly increased (p<0.01) and 6-keto-PGF1alpha; markedly decreased (p < 0.01), but there was no significant change in TXB2 and PGE2. OxyHb did not induce femoral arterial spasm. But the LPO content was...
- Subjects
CEREBRAL vasospasm; SEROTONIN; OXYHEMOGLOBIN; PROSTAGLANDINS; FREE radicals; RABBITS; CEREBROSPINAL fluid; BLOOD plasma; RADIOIMMUNOASSAY; CEREBRAL arteries
- Publication
Vascular Surgery, 1993, Vol 27, Issue 1, p1
- ISSN
0042-2835
- Publication type
Article
- DOI
10.1177/153857449302700101