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- Title
Nuclear - cytoplasmic diversity and population structure of European wild Brassica oleracea.
- Authors
XU Kun; LU Guang-yuan; WU Xiao-ming; GAO Gui-Zhen; CHEN Bi-yun; LV Pei-jun
- Abstract
To understand the genetic diversity and population structure of wild Brassica oleracea, 80 accessions of European B. oleraceain 7 geographic populations were analyzed. 14 representative materials as cultivated B. oleracea, B. napus and B. rapa were used as references. Chloroplast DNA was examined using 10 specific SSR primers which generated 8 polymorphic bands. According to these bands and markers, 94 materials were divided into 3 groups as B. oleracea (cultivated and wild ones), B. napus and B. rapa. They were also divided into 6 haplotypes including 2 wild B. oleracea, 3 B. napus and one B. rapa. Nuclear DNA was analyzed using 6 specific SRAP primers which generated 75 polymorphic bands. Cluster and genetic structure analys is showed that 7 eco-geographical groups of wild B. oleracea group F, D and G were clustered into 3 independent subclasses. E fell in to G subclass, while A, B, C group were mixed with each other. The Nei's genome diversity index(H) and Shannon's index (S) of wild B. oleracea were 0.3013 and 0.4611 respectively. Analys is of molecular variance (AMOVA) showed that the variance of intra-population components was 80%, and that of inter-population was 20%.It suggested that the European wild B. olerace a tested had rich genetic diversity.
- Subjects
PLANT population genetics; COLE crops; CYTOPLASMIC inheritance; PLANT genetics; CHLOROPLAST DNA; PLANT genes
- Publication
Chinese Journal of Oil Crop Sciences, 2011, Vol 33, Issue 2, p111
- ISSN
1007-9084
- Publication type
Article