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- Title
Analysis of the cytotoxicity and bioactivity of CeraSeal, BioRoot™ and AH Plus<sup>®</sup> sealers in pre-osteoblast lineage cells.
- Authors
de Almeida-Junior, Luciano Aparecido; de Campos Chaves Lamarque, Giuliana; Herrera, Henry; Arnez, Maya Fernanda Manfrin; Lorencetti-Silva, Francine; Silva, Raquel Assed Bezerra; Silva, Léa Assed Bezerra; Paula-Silva, Francisco Wanderley Garcia
- Abstract
Background: The objective of the present study was to evaluate in vitro the cytotoxicity and bioactivity of various endodontic sealers (CeraSeal, BioRoot™ and AH Plus®) in pre-osteoblast mouse cells (MC3T3 cells). Methods: MC3T3 cells (ATCC CRL-2594) were plated in 1 × 104 cells/well in 96-well plates in contact with endodontic sealers at concentrations of 1:10 and 1:100. Cell viability was evaluated by MTT assay after 24 and 48 h. In addition, sealer bioactivity was measured by RT-PCR for mediator of inflammation (Tnf, Ptgs2) and mineralization (Runx2, Msx1, Ssp1 and Dmp1) after 24 h and by Alizarin Red S Assay of mineralization after 28 days. Data were analyzed using one-way ANOVA followed by the Tukey's post-test at a significance level of 5%. Results: BioRoot™ presented 24-hour cytotoxicity (p < 0.05) at 1:10 concentration. In the period of 48 h, no endodontic cement was cytotoxic to the cells compared to the control (p > 0.05). TNF-α gene expression was induced by AH Plus® (p < 0.05), while Ptgs2 was induced by the CeraSeal and BioRoot™ (p < 0.05). The expression of Runx2 was stimulated by BioRoot™ and AH Plus® (p < 0.05). In contrast, the expression of Dmp-1Dmp1 was higher for the CeraSeal and BioRoot™ (p < 0.05). Nonetheless, the sealers did not impact the formation of mineralization nodules (p > 0.05). Conclusion: CeraSeal, BioRoot™ and AH Plus® sealers were not cytotoxic to MC3T3 cells within 48 h, but differentially induced the expression of genes related to inflammation and mineralization without impacting biomineralization by the cells.
- Subjects
PROTEINS; REVERSE transcriptase polymerase chain reaction; STATISTICS; ANALYSIS of variance; DENTAL materials; ANIMAL experimentation; INFLAMMATION; OSTEOBLASTS; SYNTHETIC gums &; resins; CELL physiology; CELL survival; GENE expression; TOXICITY testing; RESEARCH funding; DATA analysis; MICE; BIOMINERALIZATION
- Publication
BMC Oral Health, 2024, Vol 24, Issue 1, p1
- ISSN
1472-6831
- Publication type
Article
- DOI
10.1186/s12903-024-04021-2