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- Title
Insights into structure, affinity, specificity, and function of GAG-protein interactions through the chemoenzymatic preparation of defined sulfated oligohyaluronans.
- Authors
Schiller, Jürgen; Lemmnitzer, Katharina; Dürig, Jan-Niklas; Rademann, Jörg
- Abstract
High amounts of glycosaminoglycans (GAG) such as hyaluronan (HA) occur in connective tissues. There is nowadays increasing evidence that a "sulfation code" exists which mediates numerous GAG functions. High molecular weight and inhomogeneity of GAG, however, aggravated detailed studies. Thus, synthetic oligosaccharides were urgently required. We will review here chemoenzymatic and analytic strategies to provide defined sulfated and anomerically modified GAG oligosaccharides of the HA type. Representative studies of protein/GAG interactions by (bio)chemical and biophysical methods are reported yielding novel insights into GAG-protein binding. Finally, the biological conclusions and in vivo applications of defined sulfated GAG oligosaccharides will be discussed.
- Subjects
GLYCOSAMINOGLYCANS; MOLECULAR weights; OLIGOSACCHARIDES; SULFATION; CONNECTIVE tissues; STRUCTURE-activity relationships
- Publication
Biological Chemistry, 2021, Vol 402, Issue 11, p1375
- ISSN
1431-6730
- Publication type
Article
- DOI
10.1515/hsz-2021-0165