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- Title
Preparation and biological evaluation of radiogallium labeled glucagon for SPECT imaging.
- Authors
Jalilian, Amir Reza; Jouiaei, Mahdokht; Doroudi, Alireza; Garousi, Javad; Moradkhani, Sedigheh
- Abstract
In this work, recently prepared Ga-labeled glucagon (Ga-DTPA-GCG) for imaging studies (radiochemical purity >94%; HPLC, S.A. 296–370 GBq/mM) was used in biological studies. The wild-type rat biodistribution results, 2 h post injection, demonstrated high tissue:muscle ratios for target tissues (liver, kidney, heart, spleen, fat intestine stomach and pancreas), 234, 18.45, 7.12, 1.75, 128.7, 4.9, 6.3 and 1.11, respectively. The tracer binding capacity using freshly prepared rat brain homogenate demonstrated significant specific binding of the tracer to neuronal GCG receptors (Ga-DTPA-GCG/Ga:3 and Ga-DTPA-GCG/GaDTPA:2.2 at 90 min). SPECT images also demonstrated target specific binding of the tracer at 4 h. The data suggests the tracer is accumulated in GCGR rich tissues 2–4 h post injection, suggesting potentials of the tracer for future imaging studies in glocagonoma models.
- Subjects
GLUCAGON; LABORATORY rats; GLUCAGONOMA; DIAGNOSTIC imaging; CELL physiology
- Publication
Journal of Radioanalytical & Nuclear Chemistry, 2010, Vol 285, Issue 3, p555
- ISSN
0236-5731
- Publication type
Article
- DOI
10.1007/s10967-010-0555-z