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- Title
Characterization and immunogenicity of norovirus capsid-derived virus-like particles purified by anion exchange chromatography.
- Authors
Huhti, L.; Tamminen, K.; Vesikari, T.; Blazevic, V.
- Abstract
Recombinant baculovirus (BV) expression systems are widely applied in the production of viral capsid proteins and virus-like particles (VLPs) for use as immunogens and vaccine candidates. Traditional density gradient purification of VLPs does not enable complete elimination of BV-derived impurities, including live viruses, envelope glycoprotein gp64 and baculoviral DNA. We used an additional purification system based on ionic strength to purify norovirus (NoV) GII-4 capsid-derived VLPs. The anion exchange chromatography purification led to highly purified VLPs free from BV impurities with intact morphology. In addition, highly purified VLPs induced strong NoV-specific antibody responses in BALB/c mice. Here, we describe a method for NoV VLP purification and several methods for determining their purity, including quantitative PCR for BV DNA detection.
- Subjects
NOROVIRUSES; CAPSIDS; VIRUS-like particles; RECOMBINANT baculoviruses; IMMUNOGLOBULINS; QUANTITATIVE research
- Publication
Archives of Virology, 2013, Vol 158, Issue 5, p933
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-012-1565-7