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- Title
Resistance to Bombyx mori nucleopolyhedrovirus via overexpression of an endogenous antiviral gene in transgenic silkworms.
- Authors
Jiang, Liang; Wang, Genhong; Cheng, Tingcai; Yang, Qiong; Jin, Shengkai; Lu, Gai; Wu, Fuquan; Xiao, Yang; Xu, Hanfu; Xia, Qingyou
- Abstract
Transgenic technology is a powerful tool for improving disease-resistant species. Bmlipase-1, purified from the midgut juice of Bombyx mori, showed strong antiviral activity against B. mori nucleopolyhedrovirus (BmNPV). In an attempt to create an antiviral silkworm strain for sericulture, a transgenic vector overexpressing the Bmlipase-1 gene was constructed under the control of a baculoviral immediate early-1 (IE1) promoter. Transgenic lines were generated via embryo microinjection. The mRNA level of Bmlipase-1 in the midguts of the transgenic line was 27.3 % higher than that of the non-transgenic line. After feeding the silkworm with different amounts of BmNPV, the mortality of the transgenic line decreased to approximately 33 % compared with the non-transgenic line when the virus dose was 10 OB/larva. These results imply that overexpressing endogenous antiviral genes can enhance the antiviral resistance of silkworms.
- Subjects
SILKWORMS; NUCLEOPOLYHEDROVIRUSES; TRANSGENIC insects; VIRAL genes; GENE expression; MICROINJECTIONS; BACULOVIRUS genetics; PROMOTERS (Genetics)
- Publication
Archives of Virology, 2012, Vol 157, Issue 7, p1323
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-012-1309-8