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- Title
Characterization of Gag and Nef-specific ELISpot-based CTL responses in HIV-1 infected Indian individuals.
- Authors
Mendiratta, Sanjay; Vajpayee, Madhu; Malhotra, Uma; Kaushik, Shweta; Dar, Lalit; Mojumdar, Kamalika; Chauhan, Neeraj Kumar; Sreenivas, Vishnubhatla
- Abstract
Cytotoxic T lymphocyte (CTL) responses to Gag have been most frequently linked to control of viremia whereas CTL responses to Nef have direct relationship with viral load. IFN-γ ELISpot assay was used to screen CTL responses at single peptide level directed at HIV-1 subtype C Gag and Nef proteins in 30 antiretroviral therapy naive HIV-1 infected Indian individuals. PBMCs from 73.3% and 90% of the study population showed response to Gag and Nef antigens, respectively. The magnitude of Gag-specific CTL responses was inversely correlated with plasma viral load ( r = −0.45, P = 0.001), whereas magnitude of Nef-specific responses was directly correlated ( r = 0.115). Thirteen immunodominant regions (6 in Gag, 7 in Nef) were identified in the current study. The identification of Gag and Nef-specific responses across HIV-1 infected Indian population and targeting epitopes from multiple immunodominant regions may provide useful insight into the designing of new immunotherapy and vaccines.
- Subjects
T cells; ANTIGENS; EPITOPES; IMMUNOTHERAPY; VIRAL load; HIV
- Publication
Medical Microbiology & Immunology, 2009, Vol 198, Issue 1, p47
- ISSN
0300-8584
- Publication type
Article
- DOI
10.1007/s00430-008-0104-3