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- Title
一个云芝菌株的分离及栽培条件优化.
- Authors
夏志兰; 马鑫旺; 熊昱静; 刘飞; 谢玲
- Abstract
A wild strain of Trametes versicolor collected from rotten willow trees in Juzizhoutou, Changsha, Hunan province was isolated, identified and then domesticated. The results showed that the strain was identified as T. versicolor based on its morphological characteristics and 100% similarity between its ITS sequence (GenBank accession number: OL831142) and the published ITS sequences of T. versicolor in NCBI. The substrate for domesticated cultivation comprised 60% Eucommia ulmoides sawdust, 20% cottonseed hull, 17% wheat bran, 1% sucrose, 1% calcium carbonate and 1% gypsum. Using the above mentioned substrate and spiral bag opening method, the biological efficiency of T. versicolor reached 84.62%, and the resultant fruiting bodies contained 5.9% polysaccharides, 23.9% water-soluble extract and 3.1‰ ergosterol. The polysaccharide content and water-soluble extract content were 1.84 and 1.33 times the quality standards of the Chinese Pharmacopoeia (2020 edition), respectively.
- Subjects
CHANGSHA (Hunan Sheng, China); TRAMETES versicolor; POLYSACCHARIDES; WHEAT bran; EUCOMMIA ulmoides; FRUITING bodies (Fungi); COTTONSEED
- Publication
Acta Edulis Fungi, 2022, Vol 29, Issue 1, p48
- ISSN
1005-9873
- Publication type
Article
- DOI
10.16488/j.cnki.1005-9873.2022.01.007