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- Title
Tat-GSTpi Inhibits Dopaminergic Cells against MPP + -Induced Cellular Damage via the Reduction of Oxidative Stress and MAPK Activation.
- Authors
Choi, Yeon Joo; Yeo, Hyeon Ji; Shin, Min Jea; Youn, Gi Soo; Park, Jung Hwan; Yeo, Eun Ji; Kwon, Hyun Jung; Lee, Lee Re; Kim, Na Yeon; Kwon, Su Yeon; Kim, Su Min; Kim, Dae Won; Jung, Hyo Young; Kwon, Oh-Shin; Lee, Chan Hee; Park, Jong Kook; Lee, Keun Wook; Han, Kyu Hyung; Park, Jinseu; Eum, Won Sik
- Abstract
Glutathione S-transferase pi (GSTpi) is a member of the GST family and plays many critical roles in cellular processes, including anti-oxidative and signal transduction. However, the role of anti-oxidant enzyme GSTpi against dopaminergic neuronal cell death has not been fully investigated. In the present study, we investigated the roles of cell permeable Tat-GSTpi fusion protein in a SH-SY5Y cell and a Parkinson's disease (PD) mouse model. In the 1-methyl-4-phenylpyridinium (MPP+)-exposed cells, Tat-GSTpi protein decreased DNA damage and reactive oxygen species (ROS) generation. Furthermore, this fusion protein increased cell viability by regulating MAPKs, Bcl-2, and Bax signaling. In addition, Tat-GSTpi protein delivered into the substantia nigra (SN) of mice brains protected dopaminergic neuronal cell death in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD animal model. Our results indicate that the Tat-GSTpi protein inhibited cell death from MPP+- and MPTP-induced damage, suggesting that it plays a protective role during the loss of dopaminergic neurons in PD and that it could help to identify the mechanism responsible for neurodegenerative diseases, including PD.
- Subjects
CELL death; CHIMERIC proteins; OXIDATIVE stress; MITOGEN-activated protein kinases; PARKINSON'S disease; SUBSTANTIA nigra; GLUTATHIONE transferase
- Publication
Biomedicines, 2023, Vol 11, Issue 3, p836
- ISSN
2227-9059
- Publication type
Article
- DOI
10.3390/biomedicines11030836