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- Title
Western blot analysis of sera from dogs with suspected food allergy.
- Authors
Favrot, Claude; Linek, Monika; Fontaine, Jacques; Beco, Luc; Rostaher, Ana; Fischer, Nina; Couturier, Nicolas; Jacquenet, Sandrine; Bihain, Bernard E.
- Abstract
Background Food allergy is often suspected in dogs with clinical signs of atopic dermatitis. This diagnosis is confirmed with an elimination diet and a subsequent challenge with regular food. Laboratory tests for the diagnosis of food allergy in dogs are unreliable and/or technically difficult. Cyno- DIAL® is a Western blot method that might assist with the selection of an appropriate elimination diet. Hypothesis/Objectives To evaluate the performance of Cyno- DIAL® for the selection of an elimination diet and diagnosis of food allergy. Animals/Methods Thirty eight dogs with atopic dermatitis completed an elimination diet. Combining the results of the diet trials and the challenges, 14 dogs were classified as food allergic ( FA), 22 as nonfood-allergic and two as ambiguous cases. Results Amongst all dogs and amongst dogs with a clinical diagnosis of FA, 3% and 7% (respectively) were positive to Royal Canin Anallergenic®, Vet-Concept Kanguru® or Vet-Concept Dog Sana®; 8% and 7% to Hill's d/d Duck and Rice®; 8% and 21% to Hill's z/d Ultra Allergen Free®; 53% and 64% to Eukanuba Dermatosis FP®; and 32% and 43% to a home-cooked diet of horse meat, potatoes and zucchini. The specificity and sensitivity of Cyno- DIAL® for diagnosing food allergy were 73% and 71%, respectively. Conclusions and clinical importance Although Cyno- DIAL® was considered potentially useful for identifying appropriate foods for elimination diet trials, it cannot be recommended for the diagnosis of food allergy. The Cyno- DIAL® test performed better than some previously evaluated ELISA-based tests.
- Subjects
FOOD allergy; ATOPIC dermatitis; WESTERN immunoblotting; SKIN diseases in animals; ALLERGENS
- Publication
Veterinary Dermatology, 2017, Vol 28, Issue 2, p189
- ISSN
0959-4493
- Publication type
Article
- DOI
10.1111/vde.12412