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- Title
Single nucleotide polymorphism (SNP) marker-assisted breeding of Heterorhabditis bacteriophora for improvement of reproductive potential and stress tolerance.
- Authors
Ogaya, Christopher; Diano, Michelle Ann B.; Hategekimana, Innocent; Dörfler, Verena; Molina, Carlos; Ehlers, Ralf-Udo
- Abstract
Summary: The entomopathogenic nematode (EPN), Heterorhabditis bacteriophora , is an efficient biological control agent against several economically important insect pests. Recent research has assessed the possibility to correlate desired beneficial traits with genotype data to pave a way for marker-assisted breeding approaches. A collection of H. bacteriophora WT inbred lines has been phenotyped in this framework for stress- and virulence-related traits. However, these traits are rarely combined in a single line. Thus, unifying these traits in commercial strains is of high priority. This investigation unified beneficial traits in hybrid pools through marker-assisted breeding using single nucleotide polymorphisms (SNPs) associated with reproductive potential, longevity, virulence and cold tolerance. Recombinant inbred lines (RILs) generated from a cross between a stress tolerant WT inbred line (XX21) and a line high in in vitro reproduction potential (IL3) were genotyped via SeqSNP and screened for SNP markers associated with beneficial traits. Thereafter, a genotypic pool (X21L3) comprising 22 ILs was formed. The X21L3 pool was subsequently evaluated for the target traits in comparison with the cross parents and a commercial strain HB4. An improvement of oxidative stress tolerance at 2°C (cold tolerance) was recorded with X21L3 surviving 1 day longer than the best performing parent (XX21). The hybrid pool also survived 1 day longer than the least performing parent IL3 for the trait longevity at 25°C under oxidative stress conditions. A higher dauer juvenile (DJ) recovery (58%) and DJ yield (209 000 DJ ml−1) than the least performing parent XX21 was recorded for the pool. The storage stability in diatomaceous earth formulation at 2°C and 7.5°C was also improved by 2 and 5 days, respectively, in comparison to the least performing parent XX21. This study depicts the potential of precision marker-assisted breeding for beneficial trait improvement of H. bacteriophora.
- Subjects
SINGLE nucleotide polymorphisms; HETERORHABDITIS; DIATOMACEOUS earth; OXIDATIVE stress
- Publication
Nematology, 2024, Vol 26, Issue 3, p273
- ISSN
1388-5545
- Publication type
Article
- DOI
10.1163/15685411-bja10307