We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Nicotine metabolism and its association with CYP2A6 genotype among Indigenous people in Alaska who smoke.
- Authors
Schaefer, Krista R.; Avey, Jaedon P.; Todd, Michael R.; Beans, Julie A.; Dillard, Denise A.; Shireman, Laura M.; Thornton, Timothy A.; Tyndale, Rachel F.; Thummel, Kenneth E.; Robinson, Renee F.; Claw, Katrina G.
- Abstract
Prevalence of smoking is higher in Alaska Native and American Indian (ANAI) populations living in Alaska than the general US population. Genetic factors contribute to smoking and cessation rates. The objective of this study was to compare CYP2A6 genetic variation and CYP2A6 enzyme activity toward nicotine in an ANAI population. ANAI (N = 151) people trying to quit smoking were recruited. DNA samples were genotyped for CYP2A6 variants *1X2A, *1B, *2, *4, *9, *10, *12, and *35. Multiple nicotine metabolites were measured in plasma and urine samples, including cotinine and 3′‐hydroxycotinine used to determine CYP2A6 activity (e.g., nicotine metabolite ratio [NMR]). We calculated summary statistics for all of the genotypes and metabolites and assigned CYP2A6 activity scores based on known information. We studied the association of CYP2A6 variants with the NMR and smoking histories. The overall frequency of the CYP2A6*1B gain of function allele was high in the ANAI versus non‐ANAI populations in other studies. Both *4 null and *9 decrease of function alleles had frequencies similar to previous studies of ANAI populations. In a multivariate analysis, the genotype‐inferred CYP2A6 activity score was associated with both plasma and urine NMR (p value = 8.56E‐08 and 4.08E‐13, respectively). Plasma NMR was also associated with duration of smoking (p value < 0.01) but not urinary total nicotine equivalents uncorrected for creatinine (TNE9uc) or biological sex. Urine NMR was significantly associated (p value < 0.01) with TNE9uc. Variation in NMR in this ANAI population is explained in part by CYP2A6 genetic variation.
- Subjects
ALASKA; ALASKA Natives; NICOTINE; GENETIC variation; COTININE; SMOKE; SMOKING cessation
- Publication
CTS: Clinical & Translational Science, 2021, Vol 14, Issue 6, p2474
- ISSN
1752-8054
- Publication type
Article
- DOI
10.1111/cts.13115