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- Title
Rapid fabrication of reinforced and cell-laden vascular grafts structurally inspired by human coronary arteries.
- Authors
Terraza, Claudia; Suazo, Cristian; Zavala, Gabriela; Ocaña, Macarena; Wilkens, Camila A.; Khoury, Maroun; Acevedo, Juan Pablo; Akentjew, Tamara L.; Blaker, Jonny J.; Valenzuela, Loreto M.; Maksimcuka, Jekaterina; Vargas, Francisco; Enrione, Javier; García-Herrera, Claudio M.
- Abstract
Design strategies for small diameter vascular grafts are converging toward native-inspired tissue engineered grafts. A new automated technology is presented that combines a dip-spinning methodology for depositioning concentric cell-laden hydrogel layers, with an adapted solution blow spinning (SBS) device for intercalated placement of aligned reinforcement nanofibres. This additive manufacture approach allows the assembly of bio-inspired structural configurations of concentric cell patterns with fibres at specific angles and wavy arrangements. The middle and outer layers were tuned to structurally mimic the media and adventitia layers of native arteries, enabling the fabrication of small bore grafts that exhibit the J-shape mechanical response and compliance of human coronary arteries. This scalable automated system can fabricate cellularized multilayer grafts within 30 min. Grafts were evaluated by hemocompatibility studies and a preliminary in vivo carotid rabbit model. The dip-spinning-SBS technology generates constructs with native mechanical properties and cell-derived biological activities, critical for clinical bypass applications. Bioengineering of small diameter vascular grafts that recapitulate the features of native vessels is extremely challenging. Here the authors present a combined dip-spinning and blow-spinning technology to fabricate multi-layered cell-embedded grafts with native mechanical properties.
- Subjects
CORONARY artery bypass; CORONARY arteries; CORONARY disease; VASCULAR grafts; OPERATIVE surgery
- Publication
Nature Communications, 2019, Vol 10, Issue 1, pN.PAG
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-019-11090-3