We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Potency of hyaluronic acid from eggshell–membrane for open gingival embrasure reconstruction following orthodontic tooth movement (a histomorphological study).
- Authors
Suparwitri, Sri; Alhasyimi, Ananto
- Abstract
Background: The aim of this research was to assess the effectiveness of eggshell–membrane (ESM)-containing hyaluronic acid (HA) in the treatment of open gingival embrasure (OGE) following orthodontic tooth movement (OTM). Materials and Methods: This study is an in vivo quasi experimental research. A total of 24 Cavia cobaya were equally divided into two groups, treatment (10% HA injection) and control (phosphate-buffered saline [PBS]). A separator was inserted between mandibular incisors to induce an OGE. A volume of 20 μl of either PBS (n = 12) or ESM extract (n = 12) was locally injected within the interdental papilla. Decapitation of animals was made on day 1, 4, and 7 postinjection. The staining was done using hemotoxylin and eosin to observe angiogenesis and Mallory to observe the collagen density. Fourier-transform infrared spectroscopy (FTIR) and thin-layer chromatography (TLC) analysis were performed to detect the amount of HA available in ESM. The results were then compared with independent t-tests and the Mann–Whitney test. The level of statistical significance was set at 0.05. Results: The FTIR and TLC analysis showed that HA was successfully identified in the ESM samples. Local injection of 10% HA induced an increase of angiogenesis compared to the control group on day 1 and 4 postinjection (P < 0.05). Significant differences (P < 0.05) were also noted in the collagen density and the growth of interdental papilla on day 4 and 7 postinjection. Conclusion: ESM has the potential effect of regenerating the interdental papilla construction after OTM by increasing the collagen fiber density and inducing angiogenesis.
- Subjects
EGGS; IN vivo studies; ANIMAL experimentation; RESEARCH methodology; ORTHODONTICS; PLASTIC surgery; HYALURONIC acid
- Publication
Dental Research Journal, 2022, Vol 19, Issue 12, p107
- ISSN
1735-3327
- Publication type
Article
- DOI
10.4103/1735-3327.363566