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- Title
Detection of Animal Species in Some Meat and Meat Products by Comparatively Using DNA Microarray and Real Time PCR Methods.
- Authors
ÖZPINAR, Haydar; TEZMEN, Gündüz; GÖKÇE, İnci; Hakkı TEKİNER, İsmail
- Abstract
Determination of animal species in the meat and meat products is one of the interest of food science, it is also important for consumer rights and food safety. Increasing world population has also remarkably impacted the demand for meat and meat products. Based on this fact, issues related to safety and quality in the meat products have brought up in that manner as well. Through DNA based molecular methods are improved in food analysis, it is preferred increasingly in the control of food safety. In this study, a total of 73 samples of the meat and meat products sold in stores, meat selling markets and public bazaars located in different districts of Istanbul province were analyzed for the detection of animal species notified on the labeJ by using Chipron LCD Array Analysis System. The results showed that 39 samples (53.4%) were labelled incorrectly. Randomly selected eleven samples were corrected by lontek Fluorion Meat Species Identification Kit and FDS Detection System (Real Time PCR). Hence, it was found that the results obtained by DNA Microarray and Real Time PCR methods were identical (100%) with each other, and both methods should extensively be promoted for the detection of animal species in the meat and meat products.
- Subjects
ANIMAL species; MEAT; DNA microarrays; POLYMERASE chain reaction; CONSUMER protection; FOOD safety
- Publication
Kafkas Universitesi Veteriner Fakultesi Dergisi, 2013, Vol 19, Issue 2, p245
- ISSN
1300-6045
- Publication type
Article
- DOI
10.9775/kvfd.2012.7616