We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Expression of the 14 kDa galactose-binding protein, galectin-1, on human tubular epithelial cells.
- Authors
Bürger, Antje; Filsinger, Sabine; Cooper, Douglas N. W.; Hänsch, Getrud Maria
- Abstract
By reverse phase PCR and Northern blotting, RNA of the 14 kDa galactose-binding protein (galectin-1) could be identified in primary cultures of human tubular epithelial cells. To assess protein synthesis and the possible function of galectin-1 on TEC, the cellular proteins were biosyntheticically labeled with [35S]-methionine and adsorbed to immobilized laminin. Multiple radiolabeled proteins were eluted, a strong band in the area of 14 kDa was seen, coinciding with the galectin-1 band as identified by Western blotting. Surface expression of galectin-1 was seen by cytofluorometry with two different polyclonal antibodies to galectin-1. These data are in line with the finding that tubular epithelial cells adhere to laminin, partly in a Ca2+-independent manner.
- Subjects
PROTEINS; PROTEIN binding; GALACTOSE; EPITHELIAL cells; KIDNEY tubules; WESTERN immunoblotting
- Publication
Kidney International, 1996, Vol 50, Issue 3, p754
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.1996.373