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- Title
Characterization of platelet-activating factor synthesis in glomerular endothelial cell lines.
- Authors
Kester, Mark; Nowinski, Robert J.; Holthöfer, Harry; Marsden, Philip A.; Dunn, Michael J.
- Abstract
Platelet-activating factor synthesis in two transformed lines of glomerular endothelial cells was characterized and contrasted with platelet-activating factor production in macrovaseular-derived endothelial cells as well as with glomerular cells of mesenchymal origin. Platelet-activating factor synthesis was assessed in intact cells and in cell-free preparations. Glomerular endothelial cells constitutively synthesize bio-active alkyl-PAF, and this basal activity can be chronically augmented by various inflammatory and thrombotic agents. In contrast, thrombin-mediated platelet-activating factor formation in bovine pulmonary aortic endothelial cells as well as in glomerular mesangial cells is acute and transient. The potential role of anti-inflammatory prostanoids to function as negative feedback modulators of thrombin- or endothelin-mediated platelet-activating factor synthesis was also investigated, as the synthesis of platelet-activating factor is often associated with the formation of these prostanoids. Indomethacin augmented receptor-mediated platelet-activating factor synthesis while prostanoids of the E and I series reduced agonist-stimulated PAF synthesis. In summary, the unique capacity of glomerular endothelial cells to respond to inflammatory stimuli with sustained platelet-activating factor synthesis is a clear indication of this cell's pivotal role in augmenting the inflammatory response in the limited environment of the glomerulus.
- Subjects
BLOOD platelets; CELLS; THROMBIN; BLOOD coagulation factors; NONSTEROIDAL anti-inflammatory agents; INDOMETHACIN
- Publication
Kidney International, 1994, Vol 46, Issue 5, p1404
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.1994.412