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- Title
REACTIVIDAD DEL ANTIGENO GST-SAPA DE TRYPANOSOMA CRUZI FRENTE A SUEROS DE PACIENTES CON ENFERMEDAD DE CHAGAS Y LEISHMANIASIS.
- Authors
Gil, Jose; Cimino, Ruben; Quiroga, Ines lopez; Cajal, Silvana; Acosta, Norma; Juarez, Marisa; Zacca, Rosa; Orellana, Viviana; Krolewiecki, Alejandro; Diosque, Patricio; Nasser, Julio
- Abstract
Serologic diagnosis of Trypanosoma cruzi infection is important due to the limited sensitivity of direct parasitologic methods for diagnosis in the indeterminate and chronic phases of disease. SAPA antigen has been used in several studies and has been shown to be a good marker for use in the diagnosis of T. cruzi infection. Chagas disease and leishmaniasis are endemic in northern Salta with overlapping zones of transmission, which frequently leads to T. cruzi-Leishmania spp. mixed infections. Diagnosis is complicated by the fact that there is significant cross-reactivity when non-specific antigens are used. We evaluated the reactivity of GST-SAPA antigen in the ELISA test (ELISA-SAPA) against sera from persons infected with T. cruzi (n = 154), leishmaniasis (n = 66), mixed infections (29), and healthy controls (n = 28) using commercial ELISA and IHA kits as reference tests. For ELISA-SAPA the sensitivity, specificity and kappa index were calculated for detection of T. cruzi infection. Among sera from patients infected with leishmaniasis, 30.5% of co-infections were detected. ELISA-SAPA sensitivity was 97.1% (confidence interval 95%: 94.5-99.9), specificity was 100% (confidence interval 95%: 99.4-100), and kappa index was 96% (confidence interval 95%: 93-99%), for detection of T. cruzi infection. Sensitivity, specificity and kappa indices have shown a high efficiency of ELISA-SAPA.
- Subjects
TRYPANOSOMA cruzi; DIAGNOSIS; CHAGAS' disease; LEISHMANIASIS; ANTIGENS; INFECTION; ENZYME-linked immunosorbent assay
- Publication
Medicina (Buenos Aires), 2011, Vol 71, Issue 2, p113
- ISSN
0025-7680
- Publication type
Article