We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
High-throughput phenotyping of plant resistance to aphids by automated video tracking.
- Authors
Kloth, Karen J.; ten Broeke, Cindy J. M.; Thoen, Manus P. M.; Hanhart-van den Brink, Marianne; Wiegers, Gerrie L.; Krips, Olga E.; Noldus, Lucas P. J. J.; Dicke, Marcel; Jongsma, Maarten A.
- Abstract
Background Piercing-sucking insects are major vectors of plant viruses causing significant yield losses in crops. Functional genomics of plant resistance to these insects would greatly benefit from the availability of high-throughput, quantitative phenotyping methods. Results We have developed an automated video tracking platform that quantifies aphid feeding behaviour on leaf discs to assess the level of plant resistance. Through the analysis of aphid movement, the start and duration of plant penetrations by aphids were estimated. As a case study, video tracking confirmed the near-complete resistance of lettuce cultivar 'Corbana' against Nasonovia ribisnigri (Mosely), biotype Nr:0, and revealed quantitative resistance in Arabidopsis accession Co-2 against Myzus persicae (Sulzer). The video tracking platform was benchmarked against Electrical Penetration Graph (EPG) recordings and aphid population development assays. The use of leaf discs instead of intact plants reduced the intensity of the resistance effect in video tracking, but sufficiently replicated experiments resulted in similar conclusions as EPG recordings and aphid population assays. One video tracking platform could screen 100 samples in parallel. Conclusions Automated video tracking can be used to screen large plant populations for resistance to aphids and other piercing-sucking insects.
- Subjects
LEAF diseases &; pests; PLANT resistance to insects; APHIDS; PHENOTYPES; VIDEO recording
- Publication
Plant Methods, 2015, Vol 11, Issue 1, p1
- ISSN
1746-4811
- Publication type
Article
- DOI
10.1186/s13007-015-0044-z