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- Title
G<sub>13</sub> is an essential mediator of platelet activation in hemostasis and thrombosis.
- Authors
Moers, Alexandra; Nieswandt, Bernhard; Massberg, Steffen; Wettschureck, Nina; Grüner, Sabine; Konrad, Ildiko; Schulte, Valerie; Aktas, Barsom; Gratacap, Marie-Pierre; Simon, Melvin I; Gawaz, Meinrad; Offermanns, Stefan
- Abstract
Platelet activation at sites of vascular injury is essential for primary hemostasis, but also underlies arterial thrombosis leading to myocardial infarction or stroke. Platelet activators such as adenosine diphosphate, thrombin or thromboxane A2 (TXA2) activate receptors that are coupled to heterotrimeric G proteins. Activation of platelets through these receptors involves signaling through Gq, Gi and Gz (refs. 4-6). However, the role and relative importance of G12 and G13, which are activated by various platelet stimuli, are unclear. Here we show that lack of Ga13, but not Ga12, severely reduced the potency of thrombin, TXA2 and collagen to induce platelet shape changes and aggregation in vitro. These defects were accompanied by reduced activation of RhoA and inability to form stable platelet thrombi under high shear stress ex vivo. Ga13 deficiency in platelets resulted in a severe defect in primary hemostasis and complete protection against arterial thrombosis in vivo. We conclude that G13-mediated signaling processes are required for normal hemostasis and thrombosis and may serve as a new target for antiplatelet drugs.
- Subjects
BLOOD platelet activation; HEMOSTASIS; THROMBOSIS; MYOCARDIAL infarction; G proteins; THROMBIN
- Publication
Nature Medicine, 2003, Vol 9, Issue 11, p1418
- ISSN
1078-8956
- Publication type
Article
- DOI
10.1038/nm943