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- Title
Proteome Profiling of Human Cutaneous Leishmaniasis Lesion.
- Authors
da Silva Santos, Claire; Attarha, Sanaz; Saini, Ravi Kanth; Boaventura, Viviane; Costa, Jackson; Khouri, Ricardo; Barral-Netto, Manoel; Brodskyn, Cláudia Ida; Souchelnytskyi, Serhiy
- Abstract
In this study, we used proteomics and biological network analysis to evaluate the potential biological processes and components present in the identified proteins of biopsies from cutaneous leishmaniasis (CL) patients infected by Leishmania braziliensis in comparison with normal skin. We identified 59 proteins differently expressed in samples from infected and normal skin. Biological network analysis employing identified proteins showed the presence of networks that may be involved in the cell death mediated by cytotoxic T lymphocytes. After immunohistochemical analyses, the expression of caspase-9, caspase-3, and granzyme B was validated in the tissue and positively correlated with the lesion size in CL patients. In conclusion, this work identified differentially expressed proteins in the inflammatory site of CL, revealed enhanced expression of caspase-9, and highlighted mechanisms associated with the progression of tissue damage observed in lesions.
- Subjects
CUTANEOUS leishmaniasis; PROTEOMICS; SKIN inflammation; IMMUNOHISTOCHEMISTRY; GENE expression; CANCER invasiveness; THERAPEUTICS
- Publication
Journal of Investigative Dermatology, 2015, Vol 135, Issue 2, p400
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1038/jid.2014.396