We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Oscillatory and Pulsatile Shear Stress Differentially Influences Mitochondrial Membrane Potential.
- Authors
Rouhanizadeh, Mahsa; Meng-Tse Chen; Cadenas, Enrique; Hsiai, Tzung K.
- Abstract
Introduction: Δψm is considered an indicator of mitochondrial energy state. Reactive oxygen species (ROS) generation is known to induce mitochondrial depolarization and dysfunction. We assessed whether oscillatory (OSS) and pulsatile shear stress (PSS) differentially influenced mitochondrial membrane potential (Δψm) in bovine aortic endothelial cells (BAEC). Methods: BAEC monolayers were exposed to PSS at a mean shear stress (τave) of 23 dyn cm-2 and OSS at τave= 0.02 ± 3 dyn cm-2 in a dynamic parallel plate flow system for 1, 2 and 4 hours. MnTMPYP, a MnSOD mimetic was applied as a positive control. Tetramethylrhodamine methyl ester (TMRM), a cationic fluorescent dye was used for measurement of Δψm. Separately, mRNA level of MnSOD was quantified in response to OSS and PSS. Results: Δψm was increased by 12 ± 5.3 mV in response to OSS and by 33.5 ± 8.9 mV in response to PSS, suggesting that PSS is a more potent inducer than OSS in regulating Δψm. Furthermore, Δψm in response to MnTMPYP was increased to the same extent as responses to PSS (Fig. 1). Additionally, the differential levels of variations in Δψm were in agreement with the levels of MnSOD mRNA in response to PSS and OSS (Fig. 2), suggesting that MnSOD activities play an important role in Δψm. Conclusion: We demonstrate that PSS and OSS differentially regulates Δψm in parallel with the trends in MnSOD mRNA, implicating the important interplay between MnSOD and Δψm.
- Subjects
PHYSIOLOGICAL stress; MITOCHONDRIAL membranes; GENETIC regulation; MESSENGER RNA; SUPEROXIDE dismutase
- Publication
FASEB Journal, 2007, Vol 21, Issue 5, pA573
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fasebj.21.5.a573-c