We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Long Noncoding RNA LINC00958 Accelerates Gliomagenesis Through Regulating miR-203/CDK2.
- Authors
Guo, Erkun; Liang, Chaohui; He, Xin; Song, Guozhi; Liu, Hongjiang; Lv, Zhongqiang; Guan, Jianchao; Yang, Dezhen; Zheng, Jiapeng
- Abstract
Increasing evidence has indicated that long noncoding RNAs (lncRNAs) play crucial roles in various biological processes, including glioma. However, the underlying mechanism of lncRNAs in gliomagenesis is still ambiguous. In this study, we aim to investigate the role of long intergenic noncoding RNA 00958 (LINC00958) in the tumorigenesis of glioma. Results revealed that LINC00958 was significantly upregulated in glioma tissues and cell lines compared with that of adjacent normal brain tissues and normal human astrocytes. Moreover, the ectopic overexpression of LINC00958 was correlated with poor prognosis of glioma patients. Loss-of-function experiments indicated that LINC00958 knockdown suppressed glioma cell proliferation, invasion, and induced cycle arrest at G0/G1 phase <italic>in vitro</italic>, and inhibited tumor growth <italic>in vivo</italic>. Bioinformatics programs and luciferase reporter assay revealed that miR-203 shared complementary binding sites with both 3′-untranslated region of LINC00958 and CDK2. In summary, our study concludes that LINC00958 acts as an oncogenic gene in the gliomagenesis through miR-203-CDK2 regulation, providing a novel insight into glioma tumorigenesis.
- Subjects
GLIOMAS; NEOPLASTIC cell transformation; ASTROCYTES; CELL lines; BIOINFORMATICS; LUCIFERASES
- Publication
DNA & Cell Biology, 2018, Vol 37, Issue 5, p465
- ISSN
1044-5498
- Publication type
Article
- DOI
10.1089/dna.2018.4163