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- Title
Construction of a Biocompatible and Antioxidant Multilayer Coating by Layer-by-Layer Assembly of κ-Carrageenan and Quercetin Nanoparticles.
- Authors
Souza, Marthyna P.; Vaz, Antônio F. M.; Costa, Thacianna B.; Cerqueira, Miguel A.; De Castro, Célia M. M. B.; Vicente, António A.; Carneiro-da-Cunha, Maria G.
- Abstract
The present work aimed at the construction and characterization of a multilayer coating based on κ-carrageenan and quercetin-loaded lecithin/chitosan nanoparticles (Np) by the layer-by-layer technique and the evaluation of its antioxidant capacity and potential cytotoxicity in vitro. The multilayered coating was successfully self-assembled, as confirmed by UV-Vis spectroscopy, contact angle, atomic force microscopy (AFM), and scanning electron microscopy (SEM). Multilayered coatings showed to have antioxidant capacity, with a DPPH• radical scavenging activity of 31.32 ± 3.13% and a result of the FRAP assay of 799.41 ± 95.39 μM of ferrous ion (Fe2+) equivalent. These coatings were also shown to be devoid of cell toxicity, as evaluated by determination of nitric oxide production and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test. The alveolar macrophages culture was tested in the presence of the κ-carrageenan/quercetin-Np multilayer coating and showed a cell viability of 91.3 ± 9.6%. These results suggest that this multilayered coating is adequate for surfaces modification in view of biomedical and food industry applications.
- Subjects
CARRAGEENANS; CHITOSAN; BIOMEDICAL materials; POLYELECTROLYTES; QUERCETIN; FOOD industry
- Publication
Food & Bioprocess Technology, 2018, Vol 11, Issue 5, p1050
- ISSN
1935-5130
- Publication type
Article
- DOI
10.1007/s11947-018-2077-6