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- Title
Genotype Characterization of Newcastle Disease Virus Isolated from Commercial Chicken Flocks in West Java, Indonesia.
- Authors
Putri, Dwi Desmiyeni; Handharyani, Ekowati; Soejoedono, Retno Damajanti; Setiyono, Agus; Ika Mayasari, Ni Luh Putu; Poetri, Okti Nadia
- Abstract
Newcastle disease (ND) is a worldwide very contagious poultry disease, caused by Newcastle disease virus (NDV). Despite the vaccination, ND outbreaks in Indonesia's commercial chicken flocks have been reported regularly. Our study aimed to determine the genotype of isolates and genetic relatedness with other Indonesia's NDVs published on the GenBank. Four NDV isolates were obtained from vaccinated flocks in 2011, 2014, 2015 in West Java, Indonesia. Two NDVs belong to virulent strain and the other two belong to avirulent strain. Phylogenetic analyses of F gene revealed that NDV/Ck/BGR/11 and NDV/Ck/GS/14 belong to genotype VII sub-genotype (h) and (i); whilst NDV/Ck/CJR/15 and NDV/Ck/BGR/15 belong to genotype II. The virulent NDVs were clustered in the same genotype and closely related to earlier Indonesia's NDVs isolated in 2007, 2009 and 2010. Result of current study showed that recent NDVs of sub-genotype VIIh and VIIi circulating in commercial chicken farm in West Java, Indonesia have high similarity with NDVs isolated during 2007 and 2010 in Indonesia. Our findings may be valuable for future studies to develop improved control and diagnostic strategies of ND.
- Subjects
JAWA Barat (Indonesia); NEWCASTLE disease virus; NEWCASTLE disease; CHICKENS; MICROORGANISM phylogeny; VACCINATION
- Publication
Pakistan Veterinary Journal, 2018, Vol 38, Issue 2, p184
- ISSN
0253-8318
- Publication type
Article
- DOI
10.29261/pakvetj/2018.041