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- Title
Identification of an essential sequence for dihydroceramide C-4 hydroxylase activity of mouse DES2
- Authors
Omae, Fumio; Miyazaki, Masao; Enomoto, Ayako; Suzuki, Akemi
- Abstract
Although the amino acid sequences of mouse DES1 (MDES1) and DES2 (MDES2) have 63% sequence identity, their enzymatic characteristics are quite different. MDES1 exhibits high dihydroceramide <f>Δ4</f>-desaturase activity and very low C-4 hydroxylase activity, while MDES2 is similarly active as both a dihydroceramide <f>Δ4</f>-desaturase and a C-4 hydroxylase. We constructed several chimeras of MDES1 and MDES2 and identified a region important for C-4 hydroxylase activity in MDES2. This region contains the sequence XAFGY (X=T or A or V; Y=T or N) and occurs on the C-terminal side of the first His-box of MDES2. We confirmed the conservation of this region in DES2 family members sequenced from humans, pigs, rats, chickens, zebrafish, and Xenopus.
- Subjects
AMINO acids; POULTRY; ZEBRA danio; CHICKENS
- Publication
FEBS Letters, 2004, Vol 576, Issue 1/2, p63
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2004.08.060