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- Title
The human dynamin-related protein OPA1 is anchored to the mitochondrial inner membrane facing the inter-membrane space
- Authors
Olichon, Aurélien; Emorine, Laurent J.; Descoins, Eric; Pelloquin, Laetitia; Brichese, Laetitia; Gas, Nicole; Guillou, Emmanuelle; Delettre, Cécile; Valette, Annie; Hamel, Christian P.; Ducommun, Bernard; Lenaers, Guy; Belenguer, Pascale
- Abstract
Mutations in the OPA1 gene are associated with autosomal dominant optic atrophy. OPA1 encodes a dynamin-related protein orthologous to Msp1 of Schizosaccharomyces pombe and Mgm1p of Saccharomyces cerevisiae, both involved in mitochondrial morphology and genome maintenance. We present immuno-fluorescence and biochemical evidences showing that OPA1 resides in the mitochondria where it is imported through its highly basic amino-terminal extension. Proteolysis experiments indicate that OPA1 is present in the inter-membrane space and electron microscopy further localizes it close to the cristae. The strong association of OPA1 with membranes suggests its anchoring to the inner membrane.
- Subjects
GENETIC mutation; GREEN fluorescent protein; SACCHAROMYCES cerevisiae; GENOMES
- Publication
FEBS Letters, 2002, Vol 523, Issue 1-3, p171
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(02)02985-X