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- Title
Purification and Biochemical Properties of Multiple Xylanases from Aspergillus ochraceus Tolerant to Hg Ion and a Wide Range of pH.
- Authors
Michelin, Michele; Silva, Tony; Jorge, João; Polizeli, Maria de
- Abstract
Production of multiple xylanases, in which each enzyme has a specific characteristic, can be one strategy to achieve the effective hydrolysis of xylan. Three xylanases (xyl 1, xyl 2, and xyl 3) from Aspergillus ochraceus were purified by chromatography using diethylaminoethyl (DEAE) cellulose, Biogel P-60, and Sephadex G-100 columns. These enzymes are glycoproteins of low molecular weight with an optimum temperature at 60 °C. The glycosylation presented is apparently not related to thermostability, since xyl 3 (20 % carbohydrate) was more thermostable than xyl 2 (67 % carbohydrate). Xyl 3 was able to retain most of its activity in a wide range of pH (3.5-8.0), while xyl 1 and xyl 2 presented optimum pH of 6.0. Xyl 1 and xyl 2 were activated by 5 and 10 mM MnCl and CoCl, while xyl 3 was activated by 1 mM of the same compounds. Interestingly, xyl 2 presented high tolerance toward mercury ion. Xylanases from A. ochraceus hydrolyzed xylans of different origins, such as birchwood, oat spelt, larchwood, and eucalyptus (around 90 % or more), except xyl 2 and xyl 3 that hydrolyzed with lesser efficiency eucalyptus (66.7 %) and oat spelt (44.8 %) xylans.
- Subjects
XYLANASE biotechnology; ASPERGILLUS; GLYCOPROTEINS; MICROBIAL cultures; POLYACRYLAMIDE gel electrophoresis
- Publication
Applied Biochemistry & Biotechnology, 2014, Vol 174, Issue 1, p206
- ISSN
0273-2289
- Publication type
Article
- DOI
10.1007/s12010-014-1051-7