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- Title
Identification and Functional Analysis of the NADK Gene Family in Wheat.
- Authors
Wang, Xiang; Li, Wen-Yan; Zhang, Mao-Mao; Gao, Yin-Tao; Liu, Wen-Ting; Li, Wen-Qiang; Muhammad, Izhar; Chen, Kun-Ming
- Abstract
NAD(H) kinase (NADK) is a key enzyme for maintaining the cellular NAD(H)/NADP(H) balance and for NADP(H)-based metabolic pathways. We identified four NADK homologs, NADK1-4, from three major cereal crops, wheat, rice and maize, based on their phylogenetic relationships and gene structures. Four of the 11 NADK genes isolated from the wheat genome ( TaNADK1-4) were cloned. Subcellular localization analysis showed that TaNADK1 and 2 are located in the cytosol, TaNADK3 in chloroplasts and TaNADK4 in peroxisomes. The genes of wheat NADKs were expressed in all tissues examined and throughout development, with the same tissue specificity and developmental stage dominance. Although we found some species specificities between the orthologous and/or paralogous genes of the cereal NADKs, transcripts of these genes in wheat, rice and maize were all notably affected in the presence of one of several abiotic stresses or hormonal treatments, demonstrating their crucial roles in the abiotic stress response and hormone signaling regulation. The cereal NADKs might function in cellular NAD(H)/NADP(H) balance and NADP(H) production for redox-based enzymatic processes in plants and therefore contribute to plant stress tolerance and developmental regulation. The compartmentalization of different NADKs might play a fundamental role in the regulation of multiple biological processes.
- Subjects
WHEAT genetics; PLANT genes; KINASES; PLANT identification; PLANT phylogeny; REPRODUCTIVE isolation in plants
- Publication
Plant Molecular Biology Reporter, 2016, Vol 34, Issue 1, p118
- ISSN
0735-9640
- Publication type
Article
- DOI
10.1007/s11105-015-0904-8