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- Title
Selenium research in mammals using nuclear analytical methods and related techniques in conjunction with biochemical procedures.
- Authors
Behne, D.; Alber, D.; Kyriakopoulos, A.
- Abstract
Due to the essential functions of selenium-containing enzymes and the relationships between changes in the selenium status and diseases, the determination of the element and its compounds is of great interest. Radiotracer studies with 75Se have been valuable tools in selenium research. NAA and ICP-MS allow both total element and stable isotope measurements. ICP-MS in conjunction with chromatographic separation techniques and gel electrophoretic procedures coupled with scanning methods such as XRF, PIXE and laser ablation ICP-MS have been used in the determination of the selenium compounds. In this survey the application of these methods in selenium research is discussed with the help of examples on the regulation of the selenium metabolism and the detection and investigation of novel selenium-containing proteins.
- Subjects
SELENIUM in animal nutrition; MAMMALS; CHEMICAL speciation; ISOTOPES; ENZYMES; RADIOACTIVE tracers; SELENIUM compounds; RADIOACTIVE tracers in animal nutrition
- Publication
Journal of Radioanalytical & Nuclear Chemistry, 2004, Vol 259, Issue 3, p435
- ISSN
0236-5731
- Publication type
Article
- DOI
10.1023/B:JRNC.0000020914.98944.08