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- Title
Disruption of Genes Encoding Putative Zwitterionic Capsular Polysaccharides of Diverse Intestinal Bacteroides Reduces the Induction of Host Anti-Inflammatory Factors.
- Authors
Arnolds, Kathleen L.; Yamada, Eiko; Neff, C. Preston; Schneider, Jennifer M.; Palmer, Brent E.; Lozupone, Catherine A.
- Abstract
Bacterial zwitterionic capsular polysaccharides (ZPS), such as polysaccharide A (PSA) of the intestinal commensal Bacteroides fragilis, have been shown to modulate T cells, including inducing anti-inflammatory IL-10-secreting T regulatory cells (Tregs). We previously used a genomic screen to identify diverse host-associated bacteria with the predicted genetic capacity to produce ZPSs related to PSA of B. fragilis and hypothesized that genetic disruption (KO) of a key functional gene within these operons would reduce the anti-inflammatory activity of these bacteria. We found that ZPS-KO bacteria in two common gut commensals, Bacteroides uniformis and Bacteroides cellulosilyticus, had a reduced ability to induce Tregs and IL-10 in stimulations of human peripheral blood mononuclear cells (PBMCs). Additionally, we found that macrophage stimulated with either wildtype B. fragilis or B. uniformis produced significantly more IL-10 than KOs, indicating a potentially novel function of ZPS of shifting the cytokine response in macrophages to a more anti-inflammatory state. These findings support the hypothesis that these related ZPS may represent a shared strategy to modulate host immune responses.
- Subjects
BACTEROIDES fragilis; MONONUCLEAR leukocytes; REGULATORY T cells; BACTEROIDES; POLYSACCHARIDES; T cells
- Publication
Microbial Ecology, 2023, Vol 85, Issue 4, p1620
- ISSN
0095-3628
- Publication type
Article
- DOI
10.1007/s00248-022-02037-1