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- Title
Albendazole enantiomeric metabolism and binding to cytosolic proteins in the liver fluke Fasciola hepatica.
- Authors
H. Solana; S. Scarcella; G. Virkel; C. Ceriani; J. Rodríguez; C. Lanusse
- Abstract
Abstract Fascioliasis causes important economic losses in ruminant species all over the world. Its control is largely based on the use of the flukicidal compound triclabendazole (TCBZ). However, its chemically related benzimidazole anthelmintic albendazole (ABZ) is being successfully used to control TCBZ-resistance flukes. This research gains some insights into the comparative molecular behaviour of both anthelmintics within the target fluke. The goals of the current work were: (i) to assess the competitive binding of ABZ and TCBZ to cytosolic proteins of F. hepatica, and (ii) to evaluate the enantioselective biotransformation of ABZ in microsomal fractions obtained from TCBZ-susceptible and TCBZ-resistant strains of the liver fluke. Cytosolic proteins from fluke specimens bound TCBZ with greater affinity (83%) than ABZ (44%) and the fraction of TCBZ bound to cytosolic proteins was not displaced by ABZ. The microsomes from both -susceptible and resistant flukes sulphoxidized ABZ into ABZ sulphoxide (ABZSO). However, this oxidative activity was 49% higher in microsomes from TCBZ-resistant flukes (P
- Subjects
FASCIOLA; BILIARY tract; LIVER flukes; BENZIMIDAZOLES
- Publication
Veterinary Research Communications, 2009, Vol 33, Issue 2, p163
- ISSN
0165-7380
- Publication type
Article
- DOI
10.1007/s11259-008-9166-3