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- Title
<italic>Amsacta moorei</italic> entomopoxvirus encodes a functional heparin-binding glycosyltransferase (AMV248).
- Authors
Inan, Cihan; Muratoglu, Hacer; Arif, Basil M.; Demirbag, Zihni
- Abstract
<italic>Amsacta moorei</italic> entomopoxvirus (AMEV) infects certain lepidopteran and orthopteran insects and is the most studied member of the genus <italic>Betaentomopoxvirus</italic>. It has been considered as a potential vector for gene therapy, a vector to express exogenous proteins and a biological control agent. One of its open reading frames, <italic>amv248,</italic> encodes a putative glycosyltransferase and is the only known attachment protein conserved in AMEV and chordopoxviruses. The ORF was successfully expressed and the protein was shown to bind soluble heparin, both in silico and in vitro. Our results also showed that, while viral infection was inhibited by soluble glycosaminoglycans (GAGs), GAG-deficient cells were more resistant to the virus. Finally, we revealed that <italic>amv248</italic> encodes an active heparin-binding glycosyltransferase which is likely to have a key role in the initiation of infection by AMEV.
- Subjects
HEPARIN; GLYCOSYLTRANSFERASES; CELL proliferation; GENE therapy; PATHOGENIC microorganisms
- Publication
Virus Genes, 2018, Vol 54, Issue 3, p438
- ISSN
0920-8569
- Publication type
Article
- DOI
10.1007/s11262-018-1561-4