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- Title
Structure and Metabolic Activity of the Gut Microbiota in Diarrhea-Predominant Irritable Bowel Syndrome Combined with Functional Dyspepsia.
- Authors
Kovaleva, Aleksandra; Poluektova, Elena; Maslennikov, Roman; Zolnikova, Oxana; Shifrin, Oleg; Kudryavtseva, Anna; Krasnov, George; Fedorova, Maria; Karchevskaya, Anna; Ivashkin, Vladimir
- Abstract
Gut dysbiosis presents in many digestive diseases. The aim of this study is to investigate the composition of the gut microbiota and its metabolic activity in patients with diarrhea-predominant irritable bowel syndrome combined with functional dyspepsia (I + D). This study included 60 patients with I + D and 20 healthy controls. Gut microbiota composition was studied using 16S rRNA gene sequencing. The short-chain fatty acids (SCFAs) spectrum was determined via gas–liquid chromatography. Patients with I + D had an increase in the abundance of Holdemanella, Erysipelotrichaceae, Erysipelotrichales, Prevotellaceae, Agathobacter, Slackia, Lactococcus, Pseudomonadaceae, Stenotrophomonas, Xanthomonadaceae, Rhizobiaceae, Erysipelatoclostridiaceae, Lachnospiraceae, and other taxa in addition to a decrease in the abundance of Frisingicoccus, Ralstonia, Burkholderiaceae, Hungatella, Eisenbergiella, Parabacteroides, Peptostreptococcaceae, Merdibacter, Bilophila, Rikenellaceae, Tannerellaceae, Bacteroidaceae, and Flavonifractor in comparison to controls. Patients with I + D showed significantly higher total SCFA content in feces; increased absolute content of acetic acid, propionic acid, butyric acid, and isoacids; and a significant negative shift in the anaerobic index. The relative levels of the main SCFAs and isoacids in the patient group did not differ significantly from those in the control group. The fecal acetate and isoacid levels correlated with the severity of diarrhea. The fecal butyrate level correlated with the severity of flatulence.
- Subjects
RNA metabolism; FECAL analysis; STATISTICS; DIARRHEA; SEQUENCE analysis; GUT microbiome; IRRITABLE colon; MICROBIOLOGY; PHENOMENOLOGICAL biology; MANN Whitney U Test; FISHER exact test; T-test (Statistics); GAS chromatography; PROPIONIC acid; DESCRIPTIVE statistics; ACETIC acid; DATA analysis software; DATA analysis; INDIGESTION; SHORT-chain fatty acids; BUTYRIC acid; FLATULENCE
- Publication
Gastrointestinal Disorders, 2023, Vol 5, Issue 3, p296
- ISSN
2624-5647
- Publication type
Article
- DOI
10.3390/gidisord5030024