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- Title
阿尔茨海默病小鼠模型中TRPC3通过上调ZO-1和 LRP-1蛋白表达清除β淀粉样蛋白沉积.
- Authors
张舒蕾; 张丽艳; 李珍慧; 安一鸣; 高翔宇
- Abstract
Objective To investigate the relationship between transient receptor potential canonical 3 (TRPC3) and blood-brain barrier (BBB) in the clearance of β-amyloid protein (Aβ) deposition in a mouse model of Alzheimer disease (AD). Methods Sixty mice were randomly divided into sham operation, model, 1-oleoyl-2-acetyl-sn-glycerol(OAG), and ethyl-1- (4- (2, 3, 3-trichloroacrylamide) phenyl) -5-(trifluoromethyl) -1H-pyrazole-4-carboxylate (Pyr3) groups (n = 15 per group). The Alzheimer’s disease mouse model was established by injecting Aβ1-42 into the lateral ventricle. Mice in the OAG and Pyr3 groups were injected intraperitoneally with TRPC3 agonist and inhibitor for 21 days. Morris water maze was used to detect the mice’s behavior. Evans blue staining was used to detect the permeability of the BBB. Western blotting was used to detect the expression of zonula occluden-1 (ZO-1) and low density lipoprotein receptor-related protein-1 (LRP-1). Enzyme-linked immunosorbent assay was used to determine Aβ1-42 concentration in the hippocampus and serum. Results Compared with the sham operation group, the learning and memory ability of the mice decreased in the model group, and the content of Evans blue increased. The expression of ZO-1 and LRP-1 proteins decreased, and the concentration of Aβ in brain tissue and serum increased. Compared with the model group, the learning and memory ability of the mice improved in the OAG group. Evans blue content decreased, and the expression of ZO-1 and LRP-1 proteins increased. The concentration of Aβ decreased in the brain tissue and increased in serum. Conclusion In AD mice, TRPC3 may improve the behavioral symptoms of AD by restoring BBB permeability and function, thereby upregulating the expression of ZO-1 and LRP-1 proteins, and accelerating the clearance of Aβ deposition in brain tissue.
- Subjects
ENZYME-linked immunosorbent assay; ALZHEIMER'S disease; LEARNING ability; BLOOD-brain barrier; LABORATORY mice; INTRAPERITONEAL injections
- Publication
Journal of China Medical University, 2022, Vol 51, Issue 12, p1116
- ISSN
0258-4646
- Publication type
Article
- DOI
10.12007/j.issn.0258-4646.2022.12.011