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- Title
XA21-mediated resistance to Xanthomonas oryzae pv. oryzae is dose dependent.
- Authors
Zhang, Nan; Dong, Xiaoou; Jain, Rashmi; Ruan, Deling; de Araujo Junior, Artur Teixeira; Li, Yan; Lipzen, Anna; Martin, Joel; Barry, Kerrie; Ronald, Pamela C.
- Abstract
The rice receptor kinase XA21 confers broad-spectrum resistance to Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of rice bacterial blight disease. To investigate the relationship between the expression level of XA21 and resulting resistance, we generated independent HA-XA21 transgenic rice lines accumulating the XA21 immune receptor fused with an HA epitope tag. Whole-genome sequence analysis identified the T-DNA insertion sites in sixteen independent T0 events. Through quantification of the HA-XA21 protein and assessment of the resistance to Xoo strain PXO99 in six independent transgenic lines, we observed that XA21-mediated resistance is dose dependent. In contrast, based on the four agronomic traits quantified in these experiments, yield is unlikely to be affected by the expression level of HA-XA21. These findings extend our knowledge of XA21-mediated defense and contribute to the growing number of well-defined genomic landing pads in the rice genome that can be targeted for gene insertion without compromising yield.
- Subjects
XANTHOMONAS oryzae; RICE diseases &; pests; XANTHOMONAS; WHOLE genome sequencing; TRANSGENIC rice; BACTERIAL diseases; SEQUENCE analysis
- Publication
PeerJ, 2024, p1
- ISSN
2167-8359
- Publication type
Article
- DOI
10.7717/peerj.17323