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- Title
Expression of Exogenous Human Hepatic Nuclear Factor-1α by a Lentiviral Vector and Its Interactions with Plasmodium falciparum Subtilisin-Like Protease 2.
- Authors
Shunyao Liao; Yunqiang Liu; Bing Zheng; Pyo Yun Cho; Hyun Ok Song; Yun-Seok Lee; Suk-Yul Jung; Hyun Park
- Abstract
The onset, severity, and ultimate outcome of malaria infection are influenced by parasite-expressed virulence factors as well as by individual host responses to these determinants. In both humans and mice, liver injury follows parasite entry, persisting to the erythrocytic stage in the case of infection with the fatal strain of Plasmodium falciparum. Hepatic nuclear factor (HNF)-1α is a master regulator of not only the liver damage and adaptive responses but also diverse metabolic functions. In this study, we analyzed the expression of host HNF-1α in relation to malaria infection and evaluated its interaction with the 5'-untranslated region of subtilisin-like protease 2 (subtilase, Sub2). Recombinant human HNF-1α expressed by a lentiviral vector (LV HNF-1α) was introduced into mice. Interestingly, differences in the activity of the 5'-untranslated region of the Pf-Sub2 promoter were detected in 293T cells, and LV HNF-1α was observed to influence promoter activity, suggesting that host HNF-1α interacts with the Sub2 gene.
- Subjects
GENE expression; PLASMODIUM falciparum; PROTEOLYTIC enzymes; LIVER cells; SUBTILISINS; LABORATORY mice; MICROBIAL virulence
- Publication
Korean Journal of Parasitology, 2011, Vol 49, Issue 4, p431
- ISSN
0023-4001
- Publication type
Article
- DOI
10.3347/kjp.2011.49.4.431