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- Title
Performances of rapid and connected salivary RT-LAMP diagnostic test for SARS-CoV-2 infection in ambulatory screening.
- Authors
Schneider, Francisco Santos; Molina, Laurence; Picot, Marie-Christine; L'Helgoualch, Nicolas; Espeut, Julien; Champigneux, Pierre; Alali, Mellis; Baptiste, Julie; Cardeur, Lise; Carniel, Christophe; Davy, Martin; Dedisse, Daniel; Dubuc, Benjamin; Fenech, Hugo; Foulongne, Vincent; Gaillard, Carole Fruchart; Galtier, Florence; Makinson, Alain; Marin, Grégory; Santos, Raissa Medina
- Abstract
In the context of social events reopening and economic relaunch, sanitary surveillance of SARS-CoV-2 infection is still required. Here, we evaluated the diagnostic performances of a rapid, extraction-free and connected reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay on saliva. Nasopharyngeal (NP) swabs and saliva from 443 outpatients were collected simultaneously and tested by reverse-transcription quantitative PCR (RT-qPCR) as reference standard test. Seventy-one individuals (16.0%) were positive by NP and/or salivary RT-qPCR. Sensitivity and specificity of salivary RT-LAMP were 85.9% (95%CI 77.8–94.0%) and 99.5% (98.7–100%), respectively. Performances were similar for symptomatic and asymptomatic participants. Moreover, SARS-CoV-2 genetic variants were analyzed and no dominant mutation in RT-LAMP primer region was observed during the period of the study. We demonstrated that this RT-LAMP test on self-collected saliva is reliable for SARS-CoV-2 detection. This simple connected test with optional automatic results transfer to health authorities is unique and opens the way to secure professional and social events in actual context of economics restart.
- Subjects
MEDICAL screening; DIAGNOSIS methods; SALIVA analysis; GENETIC variation; SARS-CoV-2; SALIVA
- Publication
Scientific Reports, 2022, Vol 12, Issue 1, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/s41598-022-04826-7