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- Title
Combining Direct PCR Technology and Capillary Electrophoresis for an Easy-to-Operate and Highly Sensitive Infectious Disease Detection System for Shrimp.
- Authors
Lin, Hung-Yun; Yen, Shao-Chieh; Tsai, Shou-Kuan; Shen, Fan; Lin, John Han-You; Lin, Han-Jia
- Abstract
Infectious diseases are considered the greatest threat to the modern high-density shrimp aquaculture industry. Specificity, rapidity, and sensitivity of molecular diagnostic methods for the detection of asymptomatic infected shrimp allows preventive measures to be taken before disease outbreaks. Routine molecular detection of pathogens in infected shrimp can be made easier with the use of a direct polymerase chain reaction (PCR). In this study, four direct PCR reagent brands were tested, and results showed that the detection signal of direct PCR in hepatopancreatic tissue was more severely affected. In addition, portable capillary electrophoresis was applied to improve sensitivity and specificity, resulting in a pathogen detection limit of 25 copies/PCR-reaction. Juvenile shrimp from five different aquaculture ponds were tested for white spot syndrome virus infection, and the results were consistent with the Organization for Animal Health's certified standard method. Furthermore, this methodology could be used to examine single post larvae shrimp. The overall detection time was reduced by more than 58.2%. Therefore, the combination of direct PCR and capillary electrophoresis for on-site examination is valuable and has potential as a suitable tool for diagnostic, epidemiological, and pathological studies of shrimp aquaculture.
- Subjects
WHITE spot syndrome virus; CAPILLARY electrophoresis; COMMUNICABLE diseases; SHRIMPS; SHRIMP culture; SHRIMP industry; VIRUS diseases
- Publication
Life (2075-1729), 2022, Vol 12, Issue 2, pN.PAG
- ISSN
2075-1729
- Publication type
Article
- DOI
10.3390/life12020276