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- Title
Suppressor tRNAs at the interface of genetic code expansion and medicine.
- Authors
Awawdeh, Aya; Radecki, Alexander A.; Vargas-Rodriguez, Oscar
- Abstract
Suppressor transfer RNAs (sup-tRNAs) are receiving renewed attention for their promising therapeutic properties in treating genetic diseases caused by nonsense mutations. Traditionally, sup-tRNAs have been created by replacing the anticodon sequence of native tRNAs with a suppressor sequence. However, due to their complex interactome, considering other structural and functional tRNA features for design and engineering can yield more effective sup-tRNA therapies. For over 2 decades, the field of genetic code expansion (GCE) has created a wealth of knowledge, resources, and tools to engineer sup-tRNAs. In this Mini Review, we aim to shed light on how existing knowledge and strategies to develop sup-tRNAs for GCE can be adopted to accelerate the discovery of efficient and specific sup-tRNAs for medical treatment options. We highlight methods and milestones and discuss how these approaches may enlighten the research and development of tRNA medicines.
- Subjects
GENETIC code; TRANSFER RNA; MEDICAL coding; NONSENSE mutation; STOP codons; ENGINEERING design
- Publication
Frontiers in Genetics, 2024, p1
- ISSN
1664-8021
- Publication type
Article
- DOI
10.3389/fgene.2024.1420331