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- Title
Allogeneic induction of thromboplastin synthesis in monocytes and endothelial cells. Biphasic effect of cyclosporin A.
- Authors
Carlsen, E.; Gaudernack, G.; Filion-Myklebust, C.; Pettersen, K. S.; Prydz, H.
- Abstract
Monocytes and endothelial cells were stimulated in co-culture with allogeneic lymphocytes to produce thromboplastin (TPL), The induction was biphasic. an early response (8-24 h) was greatly augmented by cyclosporin A (CS) (0.5-5 μg/ml) whereas the late response (day 3-4) was inhibited. Prednisolone inhibited both responses. Both drugs inhibited lymphocyte proliferation. Interferon-y decreased MLC TPL activity but increased thymidine incoporation. CD4+ cells were instrumental in inducing the early TPL peak in monocytes, whereas CD8+ cells decreased the TPL effect. With endothelial cells both T cell classes were equally effective. Conditioned medium from MLC as well as from co-cultures of endothelial cells and lymphocytes induced early TPL synthesis in endothelial cells. Upon allogeneic stimulation monocytes, but not endothelial cells, produced a significant amount of F-VII, most of which was apparently undercarboxylated.
- Subjects
MONOCYTES; LYMPHOCYTES; THROMBOPLASTIN; CYCLOSPORINE; INTERFERONS
- Publication
Clinical & Experimental Immunology, 1989, Vol 76, Issue 3, p428
- ISSN
0009-9104
- Publication type
Article