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- Title
Cloning of two splice variants of the rice PTS1 receptor, OsPex5pL and OsPex5pS, and their functional characterization using pex5-deficient yeast and Arabidopsis.
- Authors
Lee, Jung Ro; Jang, Ho Hee; Park, Jin Ho; Jung, Ji Hyun; Lee, Seung Sik; Park, Soo Kwon; Chi, Yong Hun; Moon, Jeong Chan; Lee, Young Mee; Kim, Sun Young; Kim, Jae-Yean; Yun, Dae-Jin; Cho, Moo Je; Lee, Kyun Oh; Lee, Sang Yeol
- Abstract
Using the rice PEX14 cDNA as a bait in a yeast two-hybrid assay, two splice variants of the type I peroxisomal targeting signal (PTS1) receptor, OsPex5pL and OsPex5pS, were cloned from a pathogen-treated rice leaf cDNA library. The proteins were produced from a single gene by alternative splicing, which generated a full-length variant, OsPEX5L, and a variant that lacked exon 7, OsPEX5S. OsPex5pL contained 11 copies of the pentapeptide motif WXXXF/Y in its N-terminus, and seven tetratricopeptide repeats in its C-terminus. Expression of OsPEX5L and OsPEX5S predominantly occurred in leaf tissues, and was induced by various stresses, such as exposure to the pathogen Magnaporthe grisea, and treatment with fungal elicitor, methyl viologen, NaCl or hydrogen peroxide. The Arabidopsis T-DNA insertional pex5 mutant, Atpex5, which does not germinate in the absence of sucrose and was resistant to indole-3-butyric acid (IBA), was perfectly rescued by over-expression of OsPex5pL, but not by OsPex5pS. Using transient expression of OsPex5pL and OsPex5pS in the Atpex5 mutant, we show that OsPex5pL translocates both PTS1- and PTS2-containing proteins into the peroxisome by interacting with OsPex7p, whereas OsPex5pS is involved only in PTS1-dependent import in Arabidopsis.
- Subjects
RICE genetics; PLANT genetic engineering; PLANT genetics; ARABIDOPSIS; PLANT biotechnology
- Publication
Plant Journal, 2006, Vol 47, Issue 3, p457
- ISSN
0960-7412
- Publication type
Article
- DOI
10.1111/j.1365-313X.2006.02797.x