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- Title
Association Between Vitamin D Metabolism Gene Polymorphisms and Risk of Tunisian Adults’ Asthma.
- Authors
Lahmar, Oussama; Salhi, Mariem; Kaabachi, Wajih; Berraies, Anissa; Ammar, Jamel; Soomro, Munawar Hussain; Larsen, Martin; Annesi-Maesano, Isabella; Hamzaoui, Kamel; Hamzaoui, Agnes
- Abstract
Introduction: Several studies have shown a strong correlation between the serum vitamin D level and asthma severity and deficits in lung function.Objective: Study the relationship between vitamin D and the severity of asthma by targeting five SNPs of vitamin D metabolism gene pathway in a Tunisian adult asthmatics population.Methods: Our case-control study includes 154 adult asthmatic patients and 154 healthy Tunisian subjects. We genotyped many variants in three human genes encoding key components of the vitamin D metabolism, CYP2R1, CYP27B1, GC. The GC gene rs4588 and rs7041 polymorphisms were analysed using the PCR-RFLP method, while rs10741657 and rs12794714 for CYP2R1 gene and rs10877012 of CYP27B1 gene were investigated using TaqMan PCR genotyping techniques.Results: We found that the presence of at least one copy of the rs12794714 A, allele was associated with lower risk of developing asthma (OR 0.61). Further, the rs12794714 is a protector factor against asthma severity (OR 0.5). However, the presence of rs10877012 TG genotype is a risk factor related to asthma severity (OR 1.89). When we classified the population according to sex, our results showed that rs10877012 TT genotype was a risk factor for women subjects (OR 6.7). Moreover, the expression of TT genotype was associated with a higher risk of asthma in non-smoker patients (OR 7.13). We found a significant lower VD serum levels in asthmatics than controls but no impact of the polymorphisms on VD levels.Conclusions: We found that rs12794714 and rs10877012 SNPs were associated with asthma risk.
- Subjects
VITAMIN D; ASTHMA; PULMONARY function tests; VITAMIN D metabolism; GENES
- Publication
Lung, 2018, Vol 196, Issue 3, p285
- ISSN
0341-2040
- Publication type
Article
- DOI
10.1007/s00408-018-0101-2