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- Title
MiR-140 Targets lncRNA DNAJC3-AS1 to Suppress Cell Proliferation in Acute Myeloid Leukemia.
- Authors
Hong Li; Kehong Bi; Saran Feng; Yan Wang; Chuansheng Zhu
- Abstract
Objectives: MiR-140 and DNAJC3-AS1 have been demonstrated to play critical roles in cancer biology, while their participation in acute myeloid leukemia (AML) is unclear. This study aimed to explore the role of miR-140 and DNAJC3-AS1 in AML. Methods: The expression of DNAJC3-AS1 and miR-140 were detected by RT-qPCR. Then, the role of DNAJC3-AS1 and miR-140 in regulating each other was explored by overexpression assay. Next, the direct interaction between DNAJC3-AS1 and miR-140 was analyzed using an RNA pulldown assay. Next, the subcellular location of DNAJC3-AS1 was explored using cellular, subcellular fractionation assay. Finally, cell proliferation analysis was evaluated with BrdU assay. Results: Increased expression levels of DNAJC3-AS1 and decreased expression levels of miR-140 were observed in AML patients. DNAJC3-AS1 was detected in nuclear and cytoplasm samples and direct interaction between DNAJC3-AS1 and miR-140 was observed. Discussion: Reduced expression levels of DNAJC3-AS1 were observed after overexpression of miR-140 in AML cells. DNAJC3-AS1 increased cell proliferation and inhibited the role of miR-140 in suppressing cell proliferation. Conclusion: In conclusion, miR-140 may target DNAJC3-AS1 to suppress cell proliferation in AML.
- Subjects
ACUTE myeloid leukemia; CELL proliferation; INHIBITION of cellular proliferation; LINCRNA; SUBCELLULAR fractionation
- Publication
Mediterranean Journal of Hematology & Infectious Diseases, 2022, Vol 14, Issue 1, p1
- ISSN
2035-3006
- Publication type
Article
- DOI
10.4084/MJHID.2022.005