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- Title
Preliminary study on early diagnosis of Alzheimer’s disease in APP/PS1 transgenic mice using multimodal magnetic resonance imaging.
- Authors
Meng Xu; Jipeng Liu; Qingguo Liu; Yu Gong; Yinyin Li; Jing Zhang; Shufeng Shi; Yuanyuan Shi
- Abstract
Alzheimer’s disease (AD) has an insidious onset and lacks clear early diagnostic markers, and by the time overt dementia symptoms appear, the disease is already in the mid-to-late stages. The search for early diagnostic markers of AD may open a critical window for Alzheimer’s treatment and facilitate early intervention to slow the progression of AD. In this study, we aimed to explore the imaging markers for early diagnosis of AD through the combined application of structural magnetic resonance imaging (sMRI), resting-state functional magnetic resonance imaging (rs-fMRI), and ¹H-magnetic resonance spectroscopy (¹HMRS) multimodal magnetic resonance imaging (MRI) techniques at the animal experimental level, with the aim to provide a certain reference for early clinical diagnosis of AD. First, sMRI scans were performed on 4-month-old amyloid beta precursor protein/presenilin 1 (APP/PS1) transgenic AD model mice and wild type mice of the same litter using a 7.0 T animal MRI scanner to analyze the differential brain regions with structural changes in the gray matter of the brain by voxel-based morphometry (VBM). Next, rs-fMRI scans were performed to analyze the differential brain regions between groups for local spontaneous brain activity and functional connectivity (FC) between brain regions. Finally, ¹HMRS scans were performed to quantify and analyze intergroup differences in the relative concentrations of different metabolites within regions of interest (cortex and hippocampus). Compared with wild type mice, the volume of the left hippocampus, and right olfactory bulb of APP/PS1 transgenic AD model mice were reduced, the functional activity of the bilateral hippocampus, right piriform cortex and right caudate putamen was reduced, the functional network connectivity of the hippocampus was impaired, and the relative content of N-acetylaspartate (NAA)in the hippocampus was decreased. In addition, this study found that imaging changes in olfactory-related brain regions were closely associated with AD diagnosis, and these findings may provide some reference for the early diagnosis of AD.
- Subjects
GENETICS of Alzheimer's disease; ALZHEIMER'S disease diagnosis; BIOMARKERS; BIOLOGICAL models; GRAY matter (Nerve tissue); HIPPOCAMPUS (Brain); NEURAL pathways; COMPUTERS; ANIMAL experimentation; MAGNETIC resonance imaging; NUCLEAR magnetic resonance spectroscopy; PROTEIN precursors; FUNCTIONAL connectivity; COMPARATIVE studies; T-test (Statistics); RESEARCH funding; DESCRIPTIVE statistics; MEMBRANE proteins; TRANSGENIC animals; DATA analysis; DATA analysis software; EARLY diagnosis; MICE
- Publication
Frontiers in Aging Neuroscience, 2024, p01
- ISSN
1663-4365
- Publication type
Article
- DOI
10.3389/fnagi.2024.1326394