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- Title
Development of real-time and quantitative QCM immunosensor for the rapid diagnosis of Aeromonas hydrophila infection.
- Authors
Hong, Sung‐Rok; Kim, Myung‐Sug; Jeong, Hyun‐Do; Hong, Suhee
- Abstract
Since bacterial infection cause a significant economic loss in fish farms, it is necessary to develop rapid diagnostic tools. Interests on label-free biosensors have been raised for the rapid detection of aquatic pathogenic bacteria but have not been extensively studied yet. Here we report a quartz crystal microbalance ( QCM) immunosensor system for the rapid and simple detection of Aeromonas hydrophila, a pathogen for fish and human, in comparison with a conventional indirect ELISA method. In QCM immunosensor system, an antibody against A. hydrophila was covalently cross-linked to the gold surface of sensor chip and bacterial attachment was monitored as real-time frequency shifts within 5 min. The frequency shifts were very positively related to the amounts of bacterial cells between 6.25 and 100 μg corresponding to 6 × 106 to 108 CFU with a high specificity. The QCM immunosensor was also able to detect bacterial cells in fish tissue extract in a dose-dependent manner. Indirect ELISA also showed the dose-dependent reaction and the amplified signal may allow a lower detection limit. However, QCM immunosensor system showed a more linear and reliable standard curve with R2 value of almost 1 (0.9999). Moreover, detection of the bacteria was much quicker and simpler.
- Subjects
FISH farming; INFECTIONS in fish; QUARTZ crystal microbalances; ENZYME-linked immunosorbent assay; BIOSENSORS
- Publication
Aquaculture Research, 2017, Vol 48, Issue 5, p2055
- ISSN
1355-557X
- Publication type
Article
- DOI
10.1111/are.13039