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- Title
CXCR2 Mediates Brucella-Induced Arthritis in Interferon γ-Deficient Mice.
- Authors
Lacey, Carolyn A.; Keleher, Lauren L.; Mitchell, William J.; Brown, Charles R.; Skyberg, Jerod A.
- Abstract
<bold>Background: </bold>Brucella species are facultative intracellular gram-negative bacteria that cause brucellosis, a common global zoonosis. Infection of the joints is the most common focal complication of brucellosis in humans. The purpose of this study was to identify mediators of focal inflammation during brucellosis.<bold>Methods: </bold>Wild-type (WT) mice are naturally resistant to Brucella infection; therefore, we infected anti-interferon γ (IFN-γ)-treated, or IFN-γ(-/-) mice with Brucella to induce osteoarticular and musculoskeletal inflammation, as we previously described. Mice were infected intraperitoneally with Brucella melitensis, and the clinical course of disease, histopathologic changes, and cytokine levels were compared among groups.<bold>Results: </bold>Rag1(-/-) mice (B- and T-cell deficient) and µMT(-/-) mice (B-cell deficient) developed paw inflammation at a similar rate and severity as WT mice following infection with B. melitensis and treatment with anti-IFN-γ. Joints from B. melitensis-infected IFN-γ(-/-) mice had markedly increased levels of CCR2 and CXCR2 ligands. While anti-IFN-γ-treated CCR2(-/-) and WT mice behaved similarly, anti-IFN-γ-treated CXCR2(-/-) or IFN-γ(-/-)/CXCR2(-/-) mice had strikingly reduced focal swelling relative to anti-IFN-γ-treated WT or IFN-γ(-/-) mice, respectively. Additionally, neutrophil recruitment was dependent on CXCR2.<bold>Conclusions: </bold>Adaptive immune cells and CCR2 are dispensable, while CXCR2 is necessary for Brucella-induced focal neutrophil recruitment and inflammation.
- Subjects
BRUCELLA; BRUCELLOSIS; GRAM-negative bacteria; JOINT infections; IMMUNITY; DRUG therapy for arthritis; CELL receptors; INFLAMMATORY mediators; ANIMAL experimentation; ARTHRITIS; INTERFERONS; MICE; RESEARCH funding; GRAM-negative aerobic bacteria; DISEASE complications; THERAPEUTICS
- Publication
Journal of Infectious Diseases, 2016, Vol 214, Issue 1, p151
- ISSN
0022-1899
- Publication type
journal article
- DOI
10.1093/infdis/jiw087