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- Title
Perlecan from human epithelial cells is a hybrid heparan/chondroitin/keratan sulfate proteoglycan
- Authors
Knox, S.; Fosang, A.J.; Last, K.; Melrose, J.; Whitelock, J.
- Abstract
Abstract: Perlecan is a multidomain proteoglycan, usually substituted with heparan sulphate (HS), and sometimes substituted with both HS and chondroitin sulphate (CS). In this paper, we describe perlecan purified from HEK-293 cells substituted with HS, CS and keratan sulphate (KS). KS substitution was confirmed by immunoreactivity with antibody 5D4, sensitivity to keratanase treatment, and fluorophore-assisted carbohydrate electrophoresis. HEK-293 perlecan failed to promote FGF-dependent cell growth in an in vitro assay. This study is the first to report perlecan containing KS, and makes perlecan one of only a very few proteoglycans substituted with three distinct types of glycosaminoglycan chains.
- Subjects
ORGANIC compounds; PHASE partition; EPITHELIAL cells; CELL culture
- Publication
FEBS Letters, 2005, Vol 579, Issue 22, p5019
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2005.07.090