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- Title
Non-redundant functionality of Lactiplantibacillus plantarum phospho-β-glucosidases revealed by carbohydrate utilization signatures associated to pbg2 and pbg4 gene mutants.
- Authors
Plaza-Vinuesa, Laura; Sánchez-Arroyo, Ana; López de Felipe, Félix; de las Rivas, Blanca; Muñoz, Rosario
- Abstract
Aim To increase our knowledge on the functionality of 6-phospho-β-glucosidases linked to phosphoenolpyruvate-dependent phosphotransferase systems (PTS) that are encountered in high redundancy in the Lactiplantibacillus plantarum WCFS1 genome. Methods and Results Two L. plantarum WCFS1 gene mutants that lacked one of the 6-phospho-β-glucosidases, ∆ pbg2 (or ∆ lp_0906) or ∆ pbg4 (or ∆ lp_2777) were constructed and the metabolic impact of these mutations assessed by high-throughput phenotyping (Omnilog). The ∆ pbg2 mutant displayed a reduced metabolic performance, having lost the capacity to utilize 20 out of 57 carbon (C)-sources used by the wild-type strain. Conversely, the ∆ pbg4 mutant conserved the capacity to metabolize most of the C-sources preferred by the wild type strain. This mutant utilized 56 C-sources albeit the range of substrates used and hence its metabolic profiling differed from that of the WCFS1 strain. The ∆ pbg2 mutant notably reduced or abolished the capacity to metabolize substrates related to pentose and glucoronate interconversions and was unable to assimilate fatty acids or nucleosides as sole C-sources for growth. The ∆ pbg4 mutant acquired the capacity to utilize efficiently glycogen, indicating an efficient supply of glucose from this source. Conclusion Lactiplantibacillus plantarum gene mutants that lack individual 6-phospho-β-glucosidases display very different carbohydrate utilization signatures showing that these enzymes can be crucial to determine the capacity of L. plantarum to consume different C-sources and hence for the nutrition and physiology of this microorganism.
- Subjects
MICROBIAL physiology; GENES; FATTY acids; NUCLEOSIDES; GLYCOGEN
- Publication
Journal of Applied Microbiology, 2023, Vol 134, Issue 4, p1
- ISSN
1364-5072
- Publication type
Article
- DOI
10.1093/jambio/lxad077